Affiliation:
1. HATAY MUSTAFA KEMAL UNIVERSITY
Abstract
This study aimed to determine food restriction effects on the profile of fatty acids and major genes on lipogenesis expressions in liver.16 Wistar albino rats were divided into two groups and different diets were given to groups for 4-weeks. First group was fed ad libitum (Control group), another group was fed the half amount of the daily requirement (Food Restriction group, FR). As well as weekly food consumption and body weight changes, total cholesterol, HDL, LDL, and triglyceride levels were determined at the end of the feeding period. In addition to the fatty acid profile, FASN and SCD-1 genes expression levels were measured in the liver. While the body weight averages decreased after 7 days and remained similar, plasma glucose levels were found lower in the FR. FASN was upregulated approximately 6 folds, and SCD-1 increased insignificantly about 3 folds in the FR. C15:0, C18:1 n9 trans, C18:2 n6 cis, C21:0, C20:2, C20:5 n3, n6 and UFA were lower, while C16:0, C18:2 n6 trans, C20:3 n6, C22:6 n3, C22:1 n9, C22:2 and SFA were higher in FR. In addition to considering the exposure time and rate of food restriction, molecular activity and interactions in other metabolic organs should be investigated.
Publisher
Kocatepe Veteriner Dergisi
Subject
General Earth and Planetary Sciences,General Environmental Science
Reference30 articles.
1. Barzilai N, Gabriely I. The role of fat depletion in the biological benefits of caloric restriction. J Nutr. 2001; 131(3): 903S-906S.
2. Bruss MD, Khambatta CF, Ruby MA, Aggarwal I, Hellerstein MK. Calorie restriction increases fatty acid synthesis and whole body fat oxidation rates. Am J Physiol Endocrinol Metab. 2010; 298(1): E108-E116.
3. Crovesy L, Masterson D, Rosado EL. Profile of the gut microbiota of adults with obesity: a systematic review. Eur J Clin Nutr. 2020; 74(9): 1251-1262.
4. Dorn C, Riener MO, Kirovski G, Saugspier M, Steib K, Weiss TS, Gäbele E, Kristiansen G, Hartmann A, Hallerbrand C. Expression of fatty acid synthase in nonalcoholic fatty liver disease. Int J Clin Exp Pathol. 2010; 3(5): 505.
5. Dos Santos BP, da Costa Diesel LF, da Silva Meirelles L, Nardi NB, Camassola M. Identification of suitable reference genes for quantitative gene expression analysis in rat adipose stromal cells induced to trilineage differentiation. Gene. 2016; 594(2): 211-219.