Isolation and Characterization of Rhizospheric Bacteria From Vuralia turcica Rhizospheric Soil

Abstract

Plant growth-promoting rhizobacteria are highly active in soil ecosystems for legumes due to their biotic activities. Vuralia turcica (Kit Tan, Vural & Kucukoduk) Uysal & Ertugrul is a Turkish endemic legume plant with potential value for ornamental and food crops. However, reports of plant growth-promoting rhizobacteria in V. turcica rhizosphere are lacking in the literature. This study includes endophytic bacterial isolation from V. turcica rhizospheric soil. The objective of this study was to isolate and characterize growth-promoting bacteria. V. turcica rhizospheric soils from four different locations of Central Anatolia of Turkey, namely L1 (Gölçayır, 38° 28´ 10.5´´ N/31° 21´ 04.4´´ E), L2 (Akşehir, 38° 28´ 17.328´´ N/31° 20´ 52.468´´ E), L3 (Dereçine, 38° 30´ 36.702´´ N/31° 17´ 56.702´´ E), L4 (Sultandağı, 38° 32´ 43.2168´´ N/31° 16´ 54.4728´´ E), were selected for bacterial isolation. Ten bacterial strains were isolated and identified through the comparison of the 16S rRNA and 16S-23S rRNA ITS region. Among them, 4 isolates belonged to Bacillus megaterium, 3 strains belonged to Stenotrophomonas rhizophila, 1 strain belonged to Rhodococcus erythropolis, 1 strain belonged to Xanthomonas albilineans, and the remaining 1 strain belonged to Lysobacter enzymogenes, respectively.