Characterization and antimicrobial properties of silver nanoparticules biosyntehized from cornelian cherry (Cornus mas L.)

Abstract

Nanoparticles produced by green synthesis has been increasingly gaining popularity, especially because they are eco-friendly and low cost. In the present article, silver nanoparticles (AgNPs) were synthesized from the extracts prepared using cornelian cherry (Cornus mas L.) at two different temperatures. The properties of obtained AgNPs were determined through UV-Vis spectroscopy, SEM, EDX, FTIR, and XRD analyses, and their antimicrobial effects on four pathogenic bacteria were investigated. The UV-Vis spectroscopy, SEM, EDX, FTIR, and XRD analysis results of the AgNPs obtained from the extraction of cherry at room temperature (20°C) and 60°C are as follows: a maximum absorption peak at 500 nm, nanoparticle sizes of 50.86 nm and 61.17 nm with spherical shapes, containing 82.82% and 83.64% Ag, C=O, and C-O, and C=O and C=C bonds, and crystal sizes of 38.81 nm and 37.88 nm, respectively. The cherry extract's antimicrobial activity was effective against Staphylococcus aureus, Listeria monocytogenes, and Salmonella Typhi, while it didn’t show activity against Escherichia coli O157:H7. The AgNPs at concentration of 25 mg/mL created inhibition zones of 9 mm, 9 mm, and 7 mm for L.monocytogenes, S.aureus, and S.Typhi, respectively, at 20°C. It was seen that 25 mg/mL AgNPs synthesized at 60°C formed 9 mm and 8 mm inhibition zones in S.aureus and L.monocytogenes cultures, respectively, whereas they showed no inhibiting activity against S.Typhi, and E.coli O157:H7. It has been seen that 20C has ease of application in two different temperatures applied in the preparation of silver nanoparticles and is a good alternative to chemical methods.