Bacterial Distribution in Teeth with Pulp Necrosis and Apical Granuloma

Abstract

ABSTRACT Aim To evaluate the distribution of bacteria in teeth with pulp necrosis and apical granuloma. Materials and methods Thirty-two extracted teeth with pulp necrosis and apical granulomas and 16 cases of isolated apical granulomas were sectioned and stained with Hematoxylin and Eosin (H&E) and Brown-Brenn staining for microscopic evaluation. The specimens were observed as to the location and distribution of bacteria involved in the process of pulp necrosis and their relationship with the periapical tooth structures and soft tissues. Results Stainable Gram-positive and Gram-negative bacteria were found in the entire root canal system. Bacterial biofilms were detected in 81.2% of cementum root canal, 46.8% in apical delta, 65.6% at the apical surface and 79.2% of the granulomas above all the specimens. Bacteria and bacterial biofilms were present in the entire root canal system of the infected teeth with apical granulomas. Conclusion Our findings suggest that bacterial infections can reach inaccessible areas to instrumentation. How to cite this article Ribeiro FC, Consolaro A, Pinheiro TN. Bacterial Distribution in Teeth with Pulp Necrosis and Apical Granuloma. Int J Experiment Dent Sci 2013;2(2):86-91.