Evaluation and Comparison of Effect of Delmopinol Application on Adherence of Candida albicans on Denture Fitting Surface on Three Types of Acrylic Resin: An in vitro Study

Abstract

ABSTRACT Purpose Denture-related stomatitis is probably the most common form of oral candidiasis and its reported prevalence varies widely ranging up to 65%. In this in vitro study, we evaluate the effect of delmopinol application on Candida albicans adherence on heat-cured acrylic resin, cold-cured acrylic resin and microwave-cured acrylic resin. Materials and methods A total of 40 specimens of each type of acrylic resin were made; 20 specimens of each type were contaminated before delmopinol treatment and 20 specimens were contaminated after delmopinol treatment. The each specimen in each tube was individually transferred to a spectrophotometer at 530 nm wavelength in order to measure the turbidity degree, through the transmittance. Aliquots of 10 μl of each tube was then collected and inoculated into agar Sabouraud plates containing 500 mmol/l of sucrose, which was incubated for 24 hours at 37oC, in order to check microbial growth. Two-way ANOVA analysis of variance test and posthoc Turkey's test were carried out to ascertain the level of significance (p < 0.001) of various observations. Results Mean of the turbidity degree of contamination after delmopinol application for heat-cured acrylic resin specimens (A1) 1440.80, colony forming units (CFU) (⨯106/ml), cold-cured acrylic resin specimens (B1) 833.30 CFU (⨯106/ml) and microwave-cured acrylic resin specimens (C1) 944.70 CFU (⨯106/ml) was significantly higher than the mean of the turbidity degree of contamination before delmopinol application for heatcured acrylic resin specimens (A2) 550.85 CFU (⨯106/ml), coldcured acrylic resin specimens (B2) 341.45 CFU (⨯106/ml) and microwave-cured acrylic resin specimens (C2) 451.50 CFU (⨯106/ml). Conclusion In case of contamination after delmopinol application, heat-cured acrylic resin showed maximum reduction in adherence of C. albicans in the study. How to cite this article Shakya P, Jain D, Rahangdale T. Evaluation and Comparison of Effect of Delmopinol Application on Adherence of Candida albicans on Denture Fitting Surface on Three Types of Acrylic Resin: An in vitro Study. Int J Prosthodont Restor Dent 2012;2(4):129-135.