Propagation of the Aujeszky’s disease virus isolate in continuous cell lines

Abstract

The objective of this study was to investigate the cultural properties of Aujeszky's disease virus isolate and the optimization of the conditions of obtaining virus-containing material in the most prospective cell cultures. As a result, it was established that the study virus isolate is highly pathogenic for laboratory animals: rabbits, guinea pigs, and rats. The following continuous cell lines were sensitive to the virus isolate: MDBK, Taurus-1 and ВНК-21. CPE (cytopathogenic effect of viruses) nature was similar in different cell cultures. The ability for propagation in cultures of continuous cell lines of the test virus isolates reached the maximum value to 4-6 passage, equaling to 6.85-7.65 lgTCD50/cm3. The obtained results show that the dynamics of the virus isolate dynamics had no significant differences, and following the adaptation, the virus isolate maintained the stable inherent propagation activity level throughout the study. As the study result, the most sensitive cell culture Taurus-1 was selected at passaging, with the highest virus accumulation observed, the infection activity was 7.55±0.12 lg TCD50/cm3. The presence of Aujeszky's disease virus was confirmed by the bioassay and the other laboratory methods, including PCR (polymerase chain reaction). The PCR method allowed defining the virus genome in the virus-containing cultural and organ and tissue material. PCR allowed amplifying the target gene fragment with the size of 194 base pairs (b.p.) in samples containing Aujeszky's disease virus DNA. No non-specific reactions were observed; PCR products did not exhibit the expected size