Chemical constituents and anti Helicobacter pylori effect of ethyl acetate fraction from Sanchezia nobilis Hook.F

Abstract

The leaves of Sanchezia Nobilis Hook. F grown in Vietnam are extracted with ethanol then distilled for ethanol retrievement under low pressure. The ethanol concentrate was shaken with n-hexane, then stirred with ethyl acetate and concentrated fractions were obtained. From the high ethyl acetate fraction, four compounds were isolated by normal phase and reversed-phase column chromatography. Their structures were determined by spectral analysis, including 1D and 2D NMR techniques (1H, 13C, DEPT, COZY, HSQC, HMBC and NOESY), High Resolution - Electrochewmical Ionization - Mass Spectroscopy (HR-ESI-MS), and identified by comparing with the recorded data. Among the isolated compounds, compound 2 (13-O-acetylfawcettimine) was first time isolated from nature; and mixture 1 (Fawcettidin), 3 (Apigenin) and 4 (Kaempferol) were first time isolated from the Sanchezia genus. The high ethyl acetate fraction of Sanchezia Nobilis Hook. F leaves were then evaluated for the anti-Helicobacter pylori (anti-HP) effect by determining the minimum inhibitory concentration method (MIC), which showed a good inhibitory, dose−dependent effect on Helicobacter pylori. The ethyl acetate fraction at 1/8 dilution showed a complete inhibitory effect on Helicobacter pylori for as long as 24 hours. At 1/128 dilution, the fraction only showed good results on inhibition after 24 hours.