Bioinformatics Studies and Examining the Tissue Distribution of Glutathione Reductase and Glucose-6-Phosphate Dehydrogenase Genes to Investigate Gender Differences in Differences in Stress Tolerance in Zebrafish (Danio rerio)

Abstract

The study aimed to investigate the bioinformatics of zebrafish glutathione reductase (gsr) and glucose-6-phosphate dehydrogenase (g6pd) genes, as well as their tissue-specific distribution. To achieve this, samples of various tissues were taken from female and male zebrafish and total RNA was extracted to obtain cDNA. qPCR was performed to determine the transcripts of gsr and g6pd genes. The structure of the genes, conserved gene maps, and phylogenetic tree were also designed. The results showed that the liver was the most dominant tissue for both gsr and g6pd genes in both female and male zebrafish. The expression of gsr gene was significantly higher in male zebrafish's liver, intestine, heart, eye, gills, and gonad tissues compared to female fish, while g6pd gene transcription was found to be significantly higher in the male liver, intestine, muscle, brain, eye, gill, kidney, stomach, and gonad tissues. Overall, this study provides valuable insights into the bioinformatics of gsr and g6pd genes in zebrafish and their tissue-specific distribution, which could help in understanding their roles in various physiological and pathological processes in zebrafish and other related species.