Determination of Lipid Lowering Properties of Methanol Extract of Tephrosia villosaAgainst High Fat Diet Induced Hyperlipidemia in Wistar Rats

Abstract

The present study was performed to determine hypolipidemic potentials of methanol extract of Tephrosia villosa against high fat diet induced hyperlipidemia in wistar rats. Defatted powdered drug of ariel parts of Tephrosia villosa was subjected to methanol extraction using soxhlet extractor. The high fat diet induced hyperlipidemia in rat model was used for the present investigation in which all experimental rats were administered with single dose of triton (250mg/kg, ip.) on 1st day with methanol extract and upto 21 days. Blood samples were collected from all the animals on the 21stday after one hour of administration of the drugs and serum lipid profile (total cholesterol, triglycerides, HDL, LDL and IDL) was estimated. All animals were sacrificed after the blood sampling and liver samples were collected. The collected liver samples were homogenized and estimated for protein and m-RNA expression ranges by western-blotting.The rats of therapeutic groups ingested with extract of Tephrosia villosaand standarddrug atorvastatin have shown significant reduction in serum cholesterol, serum triglycerides, LDL and increase in HDL indicating its ability to attenuates effect of administration of triton. Administration of methanol extract could also normalize levels of protein and m-RNA expression in therapeutic rats.The methanol extract of Tephrosia villosashown significant hypolipidemic effects against triton induced hyperlipidemia wistar rats by inhibiting cholesterol synthesis in liver.