Pırlak koyunlarında HSP90AA1 gen bölgesindeki genetik varyasyonlar

Abstract

Objective: This study aimed to reveal variations in HSP90AA1 gene region in Pırlak sheep breed. Material and Methods: A total of 100 animals randomly chosen from representative herds raised in Antalya province were genotyped by Allele-Specific Polymerase Chain Reaction (AS-PCR) technique and genotypes were validated via agarose gel electrophoresis. Results: HSP90AA1 region was found to be polymorphic in which two alleles (C and G) and three possible genotypes (CC, CG and GG) were detected. The frequencies of C and G alleles were 0.39 and 0.61, respectively, whereas genotype frequency ranged from 0.183 (CC) to 0.413 (GG). The frequency of CG genotype was calculated as 0.404. Conservation of the genetic variations at HSP90AA1 region will be required for planning selection programs against heat stress in the future. Conclusion: HSP90AA1 and similar genes may offer new opportunities to reduce heat stress caused by global climate change in the near future. Additionally, more genetic studies for the other genomic regions related to environmental stressors should be conducted in Pırlak breed in order to shape conservation studies.