Effect of HIIT and MIT on TNF-α levels and blood profile for obesity therapy

Abstract

Study purpose. Discusses the outcomes of Moderate Intensity Training (MIT) and High Intensity Interval Training (HIIT) on Tumor Necrosis Factor-alpha (TNF-α) and blood profiles to be used as a treatment option for obesity. Material and Methods. This research is a true experimental laboratory with a post-test-only control group design. Using 39 obese male Wistar rats with the distribution of 13 Wistar HIIT groups, 13 Wistar MIT, and 13 Wistar control. The exercise intervention consists of HIIT intensity (90–100% of baseline ability), MIT intensity (60-80% of baseline ability), and control without training for six weeks. A specialized rat treadmill was used for the exercises. Measuring of TNF-α levels with an enzyme-linked immunosorbent assay (ELISA). Hematology test used to determine levels of eritrosit, leukosit, trombosit. Data analysis used the Anova test. Result. TNF-α levels in MIT and HIIT were significantly higher than control levels, as indicated by P values of 0.003 (HIIT vs Control) and 0.001 (MIT vs Control). There weren’t any big differences. in the blood tests for erythrocytes, leukocytes, and platelets between groups. At body weight, HIIT is lower than the control P = 0.000, and MIT is also lower than the control P = 0.002. Conclusion. Exercise with HIIT and MIT for 6 weeks can be used as an alternative to overcome obesity because it can reduce weight obesity, but not accompanied by a decrease in TNF-α and erythrocytes, leukocytes, and platelets.