Effect of 632 nm He-Ne Laser on Gram-Positive Bacteria in Deep Partial Thickness Pediatric Burn: A Prospective Double-Blind Randomized Controlled Trial

Abstract

Background. Burns are one of the most common injuries in pediatric population, gram-positive bacteria is the main pathogens responsible for wound infection that is the leading cause of morbidity and sometimes mortality in growing children with burn. Low level laser is a new treatment option that is increasingly used in the field of wound healing. Aim. To investigate the effect of He-Ne laser on gram-positive bacteria and healing time of deep second-degree pediatric burn. Methods. Thirty children of both genders aged from 2 to 9 years old with 2nd degree upper limb burn infected with gram-positive bacteria participated in this study. They were randomly assigned into two equal groups; either the control group (group A) received systematic antibiotic and 10 minutes’ placebo laser light, or the laser group (group B) received 632 nm laser light, 6 J/cm2 scanning technique for 10 minutes. All patients received regular wound care (dressing). The study protocol was once a day, 3 times / week for one month. Pre and post-treatment evaluation of wound culture and the healing time was recorded for both groups. Results. Wound culture of all children in both groups were negative after two weeks of the study and wound healing time was significantly shorter in the laser group (14.6±5.7 days) than the control group (27±7.3 days). Conclusion. He-Ne laser is an effective option of treatment for 2nd-degree pediatric burn as it has a bactericidal effect on gram-positive bacteria and accelerates wound healing process significantly.