Optimization of Nuclear Localization Signal Composition Improves CRISPR-Cas12a Editing Rates in Human Primary Cells

Author:

Luk Kevin1ORCID,Liu Pengpeng1,Zeng Jing234,Wang Yetao56,Maitland Stacy A.1,Idrizi Feston1,Ponnienselvan Karthikeyan1,Zhu Lihua Julie157,Luban Jeremy58,Bauer Daniel E.234,Wolfe Scot A.18

Affiliation:

1. Molecular, Cell, and Cancer Biology, University of Massachusetts Chan Medical School, Worcester, Massachusetts, USA; Worcester, Massachusetts, USA.

2. Division of Hematology/Oncology, Boston Children's Hospital, Boston, Massachusetts, USA; Worcester, Massachusetts, USA.

3. Department of Pediatric Oncology, Dana-Farber Cancer Institute, Boston, Massachusetts, USA; Worcester, Massachusetts, USA.

4. Department of Pediatrics, Harvard Stem Cell Institute, Broad Institute of Harvard and MIT, Harvard Medical School, Boston, Massachusetts, USA; Worcester, Massachusetts, USA.

5. Program in Molecular Medicine, University of Massachusetts Chan Medical School, Worcester, Massachusetts, USA; Worcester, Massachusetts, USA.

6. Chinese Academy of Medical Sciences and Peking Union Medical College, Key Laboratory of Basic and Translational Research on Immune-Mediated Skin Diseases, Chinese Academy of Medical Sciences, Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs, Institute of Dermatology, Beijing, China; Worcester, Massachusetts, USA.

7. Program in Bioinformatics and Integrative Biology, University of Massachusetts Chan Medical School, Worcester, Massachusetts, USA; and Department of Worcester, Massachusetts, USA.

8. Biochemistry and Molecular Biotechnology University of Massachusetts Chan Medical School, Worcester, Massachusetts, USA.

Publisher

Mary Ann Liebert Inc

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