Generation of Nonmosaic, Two-Pore Channel 2 Biallelic Knockout Pigs in One Generation by CRISPR-Cas9 Microinjection Before Oocyte Insemination-Reference-Cited by-同舟云学术

Generation of Nonmosaic, Two-Pore Channel 2 Biallelic Knockout Pigs in One Generation by CRISPR-Cas9 Microinjection Before Oocyte Insemination

Published:2021-02-01 Issue:1 Volume:4 Page:132-146
ISSN:2573-1599
Container-title:The CRISPR Journal
language:en
Short-container-title:The CRISPR Journal

Author:

Navarro-Serna Sergio¹²,Hachem Alaa³⁴,Canha-Gouveia Analuce¹²,Hanbashi Ali³⁵,Garrappa Gabriela¹,Lopes Jordana S.¹²,París-Oller Evelyne¹²,Sarrías-Gil Lucía¹,Flores-Flores Cesar⁶,Bassett Andrew⁷,Sánchez Raul⁸,Bermejo-Álvarez Pablo⁸,Matás Carmen¹²,Romar Raquel¹²,Parrington John³,Gadea Joaquín¹²

Affiliation:

1. Department of Physiology, Faculty of Veterinary, University of Murcia, Campus Mare Nostrum, Murcia, Spain.

2. IMIB-Arrixaca, Murcia, Spain.

3. Department of Pharmacology, University of Oxford, Oxford, United Kingdom.

4. Department of Anatomy, College of Veterinary Medicine, University of Al Qadisiyah, Al Diwaniyah, Iraq.

5. Department of Pharmacology, College of Pharmacy, Jazan University, Jazan, Saudi Arabia.

6. Molecular Biology Section, Scientific and Technical Research Area (ACTI), University of Murcia, Murcia, Spain.

7. Gene Editing and Cellular Research and Development, Wellcome Sanger Institute, Cambridge, United Kingdom.

8. Department of Animal Reproduction, INIA, Madrid, Spain.

Publisher

Mary Ann Liebert Inc

Subject

Genetics,Biotechnology

Link

https://www.liebertpub.com/doi/pdf/10.1089/crispr.2020.0078

Reference58 articles.

1. Physiological roles of NAADP-mediated Ca2+ signaling

2. TPC Proteins Are Phosphoinositide- Activated Sodium-Selective Ion Channels in Endosomes and Lysosomes

3. Convergent regulation of the lysosomal two-pore channel-2 by Mg2+, NAADP, PI(3,5)P2 and multiple protein kinases

4. Calcium signaling via two-pore channels: local or global, that is the question

5. Ca2+signals, NAADP and two-pore channels: role in cellular differentiation

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