Melissa officinalis: Antibakteriyel ve Antioksidan Potansiyeli, Fenolik Profili ve Enzim Aktiviteleri
Author:
ULGEN Canan1ORCID, YILDIRIM Arzu1ORCID, TURKER Arzu1ORCID
Affiliation:
1. BOLU ABANT İZZET BAYSAL ÜNİVERSİTESİ
Abstract
Popularly referred to as lemon balm, Melissa officinalis L., has been used as a cure for gastrointestinal disorders, respiratory and cardiovascular diseases, mental and central nervous system problems, various cancers, headache, nervousness, and rheumatism. In this study, the phenolic profile, antioxidant potential, antibacterial activity, and enzyme activity of lemon balm grown in nature in Bolu, Turkey were determined. Furthermore, comparisons were made with plants grown in vitro. Individual phenolic analysis with HPLC-DAD showed that the most prevalent phenol was rosmarinic acid in both extracts and naturally-grown plants had higher amount than in vitro-grown ones. Similarly, naturally-grown plants had considerably greater levels of total phenol-flavonoid, scavenging activity for free radicals (DPPH), and phenol synthesis related enzyme (PAL). As for the enzymatic antioxidant activity (SOD and CAT), naturally-grown plants were found to have higher CAT activity and lower SOD activity. As a remarkable result, although plants grown in vitro showed moderate antibacterial activity, no effect was observed in naturally-grown plants. In general, these results showed that the M. officinalis grown in nature is exposed to more biotic and abiotic stress and increases their phenolic content remarkably and consequently antioxidant capacity.
Funder
Bolu Abant Izzet Baysal University
Publisher
KSU Journal of Agriculture and Nature
Subject
General Earth and Planetary Sciences,General Engineering,General Environmental Science
Reference46 articles.
1. Abdellatif, F., Begaa, S., Messaoudi, M., Benarfa, A, Ouakouak, H., Hassani, A, Sawicka, B. & Simal Gandara, J. (2023). HPLC–DAD analysis, antimicrobial and antioxidant properties of aromatic herb Melissa officinalis L., aerial parts extracts. Food Analytical Methods 16, 45–54. 2. Abdel-Naime, W.A., Fahim, J.R., Fouad, M.A. & Kamel, M.S. (2019). Antibacterial, antifungal, and GC–MS studies of Melissa officinalis. South African Journal of Botany 124, 228–234. 3. Adinee, J., Piri, K. & Karimi, O. (2008). Essential oil component in flower of lemon balm (Melissa officinalis L.), American Journal of Biochemistry and Biotechnology 4(3), 277–278. 4. Atanassova, M., Georgieva, S. & Ivancheva, K. (2011). Total phenolic and total flavonoid contents, antioxidant capacity and biological contaminants in medicinal herbs. Journal of the University of Chemical
Technology & Metallurgy 46(1), 81–88. 5. Barros, L., Dueñas, M., Dias, M.I., Sousa, M.J., Santos-Buelga, C. & Ferreira, I.C. (2013). Phenolic profiles of cultivated, in vitro cultured and commercial samples of Melissa officinalis L. infusions. Food Chemistry 136(1), 1–8.
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