Cuminum cyminum L. ve Myrtus communis L. Karışım Uçucu Yağının Antikandidal Aktivitesi ve Antikandidal Mekanizmasının Belirlenmesi

Abstract

C. cyminum (cumin) and M. communis (myrtle) comprise many biologically active molecules. In this study, cumin seed and myrtle leaves were mixed than hydrodistilled and analyzed in GC-MS. The main components of the essential oil were cuminal (50.71%), 1,8 cineole (8.30%), O-cymene (7.88%), β-pinene (7.62%), α-pinene (7.16%), γ-terpinene (6.09%) and α-terpinolene (2.19%). The antifungal activity of the essential oil against C. albicans, C. parapsilosis and C. tropicalis was investigated using Spectrophotometric Broth Microdilution, Colorimetric Broth Microdilution, and Agar Well Diffusion tests. Antimicrobial mechanism of the EO were researched by TTC-dehydrogenase relative activity, protein and DNA leakage analysis. While the MIC values of the oil were 3.29 mg mL-1 for C. albicans, 3.57 mg mL-1 for C. parapsilosis and 3.65 mg mL-1 for C. tropicalis, the 50% cytotoxic concentration values were between 0.17 mg/mL and 2.61 mg mL-1 for Candidas'. The inhibition values against yeasts in dark and light conditions were found as 42.1 mm on C. parapsilosis and 39 mm on C. tropicalis exposed dark and light, respectively. The inhibition zones of oil in water, glucose, glycerol and salt environment. As a result, the highest IZ was found as 26.6 mm on C. albicans and 26 mm on C. tropicalis in the presence of 3% glycerol environment. The DNA and protein level were increased when yeast were exposed to the EO. As a result, the oil of mixed C. cyminum and M. communis preserved its antimicrobial stability in different environmental conditions and should contribute to new antifungal research.