Antioxidant Effects of Some Phosphazene Derivatives in Saccharomyces cerevisiae L. Cell Culture Oxidative Stress Model

Author:

ÖZŞAHİN Ayşe Dilek1,ERDOĞDU Ayşe2,KİREÇCİ Oğuz Ayhan1,ASLAN Fatih3,İHSAN Ali4,YILMAZ Prof. Dr. Ökkeş2

Affiliation:

1. BİTLİS EREN ÜNİVERSİTESİ

2. FIRAT ÜNİVERSİTESİ

3. HARRAN ÜNİVERSİTESİ

4. OSMANİYE KORKUT ATA ÜNİVERSİTESİ

Abstract

In this study, biochemical activities of some phosphazenes were determined in Saccharomyces cerevisiae L. culture medium. Different phosphazene molecules were used in experimental practice. The experimental groups within the scope of the study were organized as control group, H2O2 (Hydrogen peroxide) and groups of phosphazen molecules. After the groups were prepared, 30 µg of phosphazen and 100 µl of H2O2 were added to other cultures, except for the control group of S. cerevisiae culture. Incubated for 72 hours in the oven at 30 °C. At the end of incubation, cell pellets were separated. Total protein levels were determined from Glutathione S-Transferase (GST) from this homogenizat. The fatty acid and lipophilic molecules were analyzed from the homogenate obtained with the hexane / isopropanol alcohol mixture. According to our experimental results, while total protein values and GST values increased in parallel with the phosphazen molecule and H2O2 added yeast cells, GST levels were decreased in some groups, although an increase in the amount of protein was observed. Ergosterol, which has an important place in the membrane structure of S. cerevisiae, was found to be higher in T3 and T3B coded phosphazenes and H2O2 groups compared to the control group, and low in T4 coded phosphazenes and H2O2 groups. Our study results revealed that as a result of the addition of phosphazen and H2O2 molecules to the culture medium of S. cerevisiae, ergosterol and fatty acid synthesis, fatty acids cause increases or decreases in the end products of enzymes that double-enter the hydrocarbon chain.

Publisher

KSU Journal of Agriculture and Nature

Subject

General Medicine

Reference23 articles.

1. Allcock HR. Morrissey WK. Winograd N 1996. Controlled Formation of Carboxylic Acid Groups at Polyphosphazene Surfaces; Oxidative and Hydrolytic Routes.Chemistry of Materials 8:2730-2738.

2. Bergman LW 2001. Growth and Maintenance of Yeast. 2001. Methods in Molecular Biology. Vol. 177. TwoHybrid Systems: Methods and Protocols Edited by: P. N. MacDonald © Humana Press Inc.. Totowa. NJ

3. Carriedo GA. Alonso FJG. Elipe PG. Brillans E. Julia L 1996. Incorparation of Stable Organic Radicals into Cyclotriphosphazene: Preparation and Charecterization of Mono – and Diradical Adducts. Journal of the Organic Letters. 3: 1625-1628.

4. Christie WW 1992. Gas Chromatography and Lipids. Glaskow. The Oil Press.

5. Civan M 2014. Bazı Trimerik Fosfazen Türevlerinin Sentezi. Kristalografik. Spektroskopik Yöntemler ile Incelenmesi ve Langmuir-Blodgett Tekniği Kullanılarak Ultra-İnce Filmlerinin Hazırlanması. Doctor of Philosophy. Department of Physics Engineering. 358.

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