Abstract
Abstract: Foot-and-mouth disease (FMD) is a highly contagious animal disease that causes devastating economic losses. The trade of live animals and derived products is only possible if the exporting country is free from disease, according to the World Organisation for Animal Health (OIE) code for FMD. One of the most important ways to prove freedom from disease is based on the measurement of antibody levels to non-structural proteins (NSP) of FMD virus in the sampled target population. For detection of the disease status of a herd, mass screening and assays such as Enzyme-linked immunosorbent assay (ELISA) and Enzyme-Linked Immunoelectrotransfer Blot Assay (EITB) were developed and described in the OIE diagnostic manual. In this study, recombinant FMDV NS proteins were produced and tested with panels of sera that were collected from both uninfected and naturally infected animals using a quantitative Western blot assay as an improved EITB which enables numerical documentation and statistical analysis. NSP band intensities were used to determine the cut-off values, differentiating infected from non-infected animals and revealing variable sensitivity among the different NSPs. The quantitative EITB results also showed a correlation with the NSP-ELISA results.
Publisher
Harran Universitesi Veteriner Fakultesi Dergisi
Subject
General Earth and Planetary Sciences,General Environmental Science
Reference24 articles.
1. Anonymous, 2011: Foot and mouth disease, Chapter 8.5. In O. T. A. H. S. Commission (ed.), Terrestrial Animal Health Code, 20th ed., OIE World Organisation for Animal Health, Paris, France.
2. Bergmann IE, Astudillo V, Malirat V, Neitzert E, 1998: Serodiagnostic strategy for estimation of foot-and-mouth disease viral activity through highly sensitive immunoassays using bioengineered nonstructural proteins. Vet Q 20: 6-9.
3. Bergmann IE, de Mello PA, Neitzert E, Beck E, Gomes I, 1993: Diagnosis of persistent aphthovirus infection and its differentiation from vaccination response in cattle by use of enzyme-linked immunoelectrotransfer blot analysis with bioengineered nonstructural viral antigens. Am J Vet Res 54: 825-831.
4. Bergmann IE, Malirat V, Neitzert E, 2005: Non-capsid proteins to identify foot-and-mouth disease viral circulation in cattle irrespective of vaccination. Biologicals 33: 235-239.
5. Bergmann IE, Malirat V, Neitzert E, Beck E, Panizzutti N, Sánchez C, Falczuk A, 2000: Improvement of a serodiagnostic strategy for foot-and-mouth disease virus surveillance in cattle under systematic vaccination: a combined system of an indirect ELISA-3ABC with an enzyme-linked immunoelectrotransfer blot assay Arch Virol 145: 473–489.