5,7,8-trihydroxyflavone Has Anticancer and Apoptotic Effects in Human Androgen-Independet Prostate Cancer PC-3 Cells

Author:

KARAKUŞ Ahmet1,ÜNAL KARAKUŞ Sevgi1ORCID,DAĞDELEN Burak2ORCID

Affiliation:

1. BARTIN ÜNİVERSİTESİ

2. SELCUK UNIVERSITY

Abstract

Natural products have been studied to provide alternative treatments against human diseases as they have various medicinal properties. One of these natural products is substances in the class of flavonoids. These bioactive molecules have antioxidant, anti-inflammatory and antitumor activities. The number of studies focusing on these molecules is increasing to discover new therapeutic agents against diseases such as cancer. We aimed to determine in-vitro the anticancer and apoptotic effects of 5,7,8-trihydroxyflavone (Nor-wogonin) flavone, which is one of these bioactive molecules and has various biological properties, on androgen-independet human prostate cancer (PC-3) cells. Nor-wogonin concentrations of 10, 20, 40, 80 µM were prepared and applied to human prostate cancer cells for 24 hours. The anticancer effect of flavone was determined by MTT 3-(4,5-dimethylthiazol-2-yl)-diphenyl tetrazolium bromide) method, and its effect on pro-poptotic and anti-apoptotic genes was determined by Real-Time PCR analysis. According to the obtained data, Nor-wogonin applied to PC-3 cells decreased in-vitro cell viability due to increasing concentration (p

Publisher

Avrasya Arastirma Gelistirme Bilim ve Teknoloji Merkezi Limited Sirketi

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