Evaluation of insecticidal and enzyme activity potentials of essential oils and extracts of Chenopodium botrys against storage products pests

Abstract

The essential oil obtained from the aerial part of Chenopodium botrys plant by steam distillation was obtained. The remaining aqueous part was extracted with the solvent ethylacetate and n-butanol, respectively. In the end, five extracts were obtained: essential oil (CB-EO), untreated aqueous extract (CB-F), ethylacetate (CB-EA), n-butanol (CB-nBu) and processed aqueous extract (CB-L). The effects of five extracts on both insecticidal (against Sitophilus granarius and Tribolium castaneum) and enzyme activities (acetylcholinesterase, xanthine oxidase (XO) and tyrosinase) were studied. It has been determined that CB-F extract has an activation effect against tyrosinase enzyme. At the end of 48 hours, CB-EA extract was determined to cause 20% death against the adult insects of Sitophilus granarius as a result of contact toxicity test. As a result of GC-MS analysis of essential oil, α-Eudesmol compound was analyzed as the main component