Identification of protein components of the transformation system in the cell line of immortalized human keratinocytes HaCaT exposed to surfactants-Reference-Cited by-同舟云学术

Identification of protein components of the transformation system in the cell line of immortalized human keratinocytes HaCaT exposed to surfactants

Published:2024 Issue:1 Volume:70 Page:61-68
ISSN:2310-6905
Container-title:Biomeditsinskaya Khimiya
language:ru
Short-container-title:BIOMED KHIM

Author:

Shkrigunov T.S.¹,Vavilov N.E.¹,Samenkova N.F.¹,Kisrieva Yu.S.¹,Rusanov A.L.¹,Romashin D.D.¹,Karuzina I.I.¹,Lisitsa A.V.¹,Petushkova N.A.¹

Affiliation:

1. Institute of Biomedical Chemistry, Moscow, Russia

Abstract

Using the method of shotgun mass spectrometry, we have evaluated changes in the proteomic profile of HaCat cells in response to the treatment with sodium dodecyl sulfate (anionic surfactant) and Triton-X100 (non-ionic surfactant) in two concentrations (12.5 µg/mL and 25.0 µg/mL). The study revealed induction of orphan CYP2S1 (biotransformation phase I) in response to Triton-X100. We have identified proteins of II (glutathione-S-transferases, GSTs) and III (solute carrier proteins, SLCs) biotransformation phases, as well as antioxidant proteins (peroxiredoxins, PRDXs; catalase, CAT; thioredoxin, TXN). Thus, proteins of all three xenobiotic detoxification phases were detected. The presented results suggest a new prospect of using HaCaT keratinocytes as a model of human epidermis for studying the metabolism of drugs/toxicants in human skin in vitro.

Publisher

Institute of Biochemistry

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