Effect of dressing with different antiseptic agents on reproductive parameters in rats undergoing abdominal incision
Author:
Akarsu G.D.12, Erbaş E.3, Akarsu S.A.4
Affiliation:
1. Division of Molecular Medicine, Laboratory for Advanced Genomics, Institut Ruđer Bošković, Zagreb, Croatia 2. Department of Pharmacy Services, Vocational School of Health Servıces, Yozgat Bozok Unıversıty, Atatürk Road 7.Km, Bilal Şahin West Campus 66100, Yozgat, Turkey 3. Department of Histology and Embryology, Faculty of Veterinary Medicine, Ataturk University, Yakutiye 25030, Erzurum, Turkey 4. Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Yakutiye 25030, Ataturk University, Erzurum, Turkey
Abstract
Antiseptic agents used in the postoperative period affect the functions of many tissues in the body, including the testicles. In this study, the effect of dressings administered with different antiseptic agents on testicular functions in rats that underwent abdominal incisions was investigated. A total of 48 Sprague-Dawley rats were used in the study. Each of the rats in the study group underwent a 4 cm-long skin and muscle operation. The incision was then stitched immediately. Antiseptics, hemp seed oil, hemp leaf oil, and cannabidiol oil were then administered to the rats for 10 days to provide antisepsis. The rats were sacrificed 24 hours after the last administration, and testicular tissues were removed. Testicular tissues were used for histopathological examination and biochemical analysis, while epididymal tissue was used for sperm analysis. According to the results, the MDA level in the antiseptic-administered group was higher than in the other experimental groups (p<0.05). Levels of SOD, CAT activities, and GSH content were found to be lower in the antiseptic group than in the hemp seed oil, hemp leaf oil, and cannabidiol oil groups (p<0.05). In testicular histology, the SEED group had the highest Johnsen score, and the antiseptic group had the lowest score (p<0.05). While JAK, P-JAK2, STAT3, PSTAT3, and NF-κB were generally higher in the antiseptic group compared to the other groups, they were lower in the SEED group. Additionally, sperm total motility rate and epididymal sperm density were highest in the SEED group (p<0.05). As a result, it was determined that cannabidiol seed oil had a good effect on testicular histology and sperm quality in male rats during the wound healing process.
Publisher
Polish Academy of Sciences Chancellery
Reference1 articles.
1. 1. Aebi H (1984) Catalase in vitro. Methods Enzymol 105: 121-126. 2. Akarsu SA, Acısu TC, Güngör İH, Cihangiroǧlu AÇ, Koca RH, Türk G, Sönmez M, Gür S, Fırat F, Duruel HE (2023) The effect of luteolin on spermatological parameters, apoptosis, oxidative stress rate in freezing rabbit semen. Pol J Vet Sci 26: 91-98. 3. Aksu EH, Kandemir FM, Küçükler S (2021) Ameliorative effect of hesperidin on streptozotocin‐diabetes mellitus‐induced testicular DNA damage and sperm quality degradation in Sprague-Dawley rats. J Food Biochem 45: e13938. 4. Aksu EH, Kandemir FM, Küçükler S (2021) The effects of hesperidin on colistin‐induced reproductive damage, autophagy, and apopto-sis by reducing oxidative stress. Andrologia 53: e13900. 5. Aksu EH, Kandemir FM, Yıldırım S, Küçükler S, Dörtbudak MB, Çağlayan C, Benzer F (2019) Palliative effect of curcumin on doxo-rubicin‐induced testicular damage in male rats. J Biochem Mol Toxicol 33: e22384. 6. Aksu E, Özkaraca M, Kandemir FM, Ömür AD, Eldutar E, Küçükler S, Çomaklı S (2016) Mitigation of paracetamol‐ -induced repro-ductive damage by chrysin in male rats via reducing oxidative stress. Andrologia 48: 1145-1154. 7. Bodnar RJ (2014) Anti-angiogenic drugs: involvement in cutaneous side effects and wound-healing complication. Adv Wound Caref 3: 635-646. 8. Carette C, Levy R, Eustache F, Baron G, Coupaye M, Msika S, Barrat C, Cohen R, Catheline JM, Brugnon F, Slim K, Barsamian C, Chevallier JM, Bretault M, Bouillot JL, Antignac JP, Rives-Lange C, Ravaud P, Czernichow S (2019) Changes in total sperm count after gastric bypass and sleeve gastrectomy: the BARIASPERM prospective study. Surg Obes Relat Dis 15: 1271-1279. 9. Carvalho RK, Rocha TL, Fernandes FH, Gonçalves BB, Souza MR, Araújo AA, Barbosa CC, Silva DM, Campos HM, Tomazett MV, Ghedini PC, Guimarães FS, Andersen ML, A Santos FC, Mazaro-Costa R (2022) Decreasing sperm quality in mice subjected to chronic cannabidiol exposure: New insights of cannabidiol-mediated male reproductive toxicity. Chem Biol Interact 351: 109743. 10. Çoban S, Ali A, Türkoğlu A R, Güzelsoy M, Üstündağ Y, Öztürk M, Demirci H (2018) Are there any ef fect of povidone iodine used in genitourinary surgery on thyroid hormones? New J Urol 13: 34-38. 11. Donkin I, Barrès R (2018) Sperm epigenetics and influence of environmental factors. Mol Metab 14: 1-11. 12. Farinon B, Molinari R, Costantini L, Merendino N (2020) The seed of industrial hemp (Cannabis sativa L.): Nutritional quality and po-tential functionality for human health and nutrition. Nutrients 12: 1935. 13. Fouda MA, Ghovanloo MR, Ruben PC (2020) Cannabidiol protects against high glucose‐induced oxidative stress and cytotoxicity in cardiac voltage‐gated sodium channels. Br J Pharmacol 177: 2932-2946. 14. Graczyk M, Lewandowska AA, Dzierżanowski T (2021) The therapeutic potential of cannabis in counteracting oxidative stress and in-flammation. Molecules 26: 4551. 15. Gur C, Akarsu, SA, Akaras N, Tuncer SC, Kandemir FM (2023) Carvacrol reduces abnormal and dead sperm counts by attenuating sodium arsenite‐induced oxidative stress, inflammation, apoptosis, and autophagy in the testicular tissues of rats. Environ Toxicol 38: 1265-1276. 16. Hassan E, Kahilo K, Kamal T, El-Neweshy M, Hassan M (2019) Protective effect of diallyl sulfide against lead-mediated oxidative damage, apoptosis and down-regulation of CYP19 gene expression in rat testes. Life Sci 226: 193-201. 17. Heymann WR (2000) Potassium iodide and the Wolff-Chaikoff effect: relevance for the dermatologist. J Am Acad Dermatol 42: 490-492. 18. Islamov R, Kustova T, Nersesyan A, Ilin A (2020) Subchronic toxicity of the new iodine complex in dogs and rats. Front Vet Sci 7: 184. 19. Johnsen SG (1970) Testicular biopsy score count – a method for registration of spermatogenesis in human testes: normal values and results in 335 hypogonadal males. Horm Res Paediatr 1: 2-25. 20. Kalkan Y, Kapakin KAT, Kara A, Atabay T, Karadeniz A, Simsek N, Karakuş E, Can I, Yıldırım S, Özkanlar S, Sengul E (2012) Protective effect of Panax ginseng against serum biochemical changes and apoptosis in kidney of rats treated with gentamicin sulphate. J Mol His 43: 603-613. 21. Karlidag R, Unal S, Sezer OH, Bay Karabulut A, Battaloğlu B, But A, Ozcan C (2006) The role of oxidative stress in postoperative de-lirium. Gen Hosp Psychiatry 28: 418-423. 22. Kiralan M, Gül V, Kara SM (2010) Fatty acid composition of hempseed oils from different locations in Turkey. Span J Agric Res 8: 385-390. 23. Koksal M, Oğuz E, Baba F, Ali Eren M, Ciftci H, Demir ME, Kurcer Z, Aral F, Ocak AR, Aksoy N, Ulas T (2012) Effects of melatonin on testis histology, oxidative stress and spermatogenesis after experimental testis ischemia-reperfusion in rats. Eur Rev Med Pharmacol Sci 16: 582-588. 24. Maccarrone M, Bab I, Bíró T, Cabral GA, Dey SK, Di Marzo V, Konje JC, Kunos G, Mechoulam R, Pacher P, Sharkey KA, Zimmer A (2015) Endocannabinoid signaling at the periphery: 50 years after THC. Trends Pharmacol Sci 36: 277-296. 25. Matkovics B (1988) Determination of enzyme activity in lipid peroxidation and glutathione pathways. Laborator Diagnos 15: 248-250. 26. Matthäus B, Brühl L (2008) Virgin hemp seed oil: An interesting niche product. Eur J Lipid Sci Technol 110: 655-661. 27. Menghini L, Ferrante C, Carradori S, D’Antonio M, Orlando G, Cairone F, Cesa S, Filippi A, Fraschetti C, Zengin G, AK G, Tacchini M, Iqbal K (2021) Chemical and bioinformatics analyses of the anti-leishmanial and anti-oxidant activities of hemp essential oil. Biomol-ecules 11: 272. 28. Noble D, Jablonka E, Joyner MJ, Müller GB, Omholt SW (2014) Evolution evolves: physiology returns to centre stage. J Physiol 592: 2237. 29. Peeters E, Spiessens C, Oyen R, De Wever L, Vanderschueren D, Miserez M (2014) Sperm motility after laparoscopic inguinal hernia repair with lightweight meshes: 3-year follow-up of a randomised clinical trial. Hernia 18: 361-367. 30. Placer ZA, Cushman LL, Johnson BC (1966) Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical sys-tems. Anal Biochem 16: 359-364. 31. Sedlak J, Lindsay RH (1968) Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman’s reagent. Anal Biochem 25: 192-205. 32. Shafiey SI, Ahmed KA, Abo-Saif AA, Abo-Youssef AM, Mohamed WR (2023) Galantamine mitigates testicular injury and disturbed spermatogenesis in adjuvant arthritic rats via modulating apoptosis, inflammatory signals, and IL-6/JAK/STAT3/SOCS3 signaling. In-flammopharmacology: doi: 10.1007/s10787-023-01268-z 33. Sun Y, Oberley LW, Li Y (1988) A simple method for clinical assay of superoxide dismutase. Clinic Chem 34: 497-500. 34. Thomas GW, Rael LT, Bar-Or R, Shimonkevitz R, Mains CW, Slone DS, Craun ML Bar-Or D (2009) Mechanisms of delayed wound healing by commonly used antiseptics. J Trauma 66: 82-91. 35. Zheng Z, Qi J, Hu L, Quyang D, Wang H, Sun Q, Lin L, You Q, Tang B (2022) A cannabidiol-containing alginate based hydrogel as novel multifunctional wound dressing for promoting wound healing. Biomater Adv 134: 112560.
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