Evaluation of uterine antioxidants in bitches suffering from cystic endometrial hyperplasia-pyometra complex
Author:
Kumar A.12, Prasad J.K.3, Verma S.4, Gattani A.5, Singh G.D.6, Singh V.K.6
Affiliation:
1. Department of Veterinary Gynaecology and Obstetrics, Bihar Veterinary College, Bihar Animal Sciences University, Patna, Bihar 800014, India 2. Department of Veterinary Biochemistry, Bihar Veterinary College, Bihar Animal Sciences University, Patna, Bihar 800014, India 3. Dean, Bihar Veterinary College, Bihar Animal Sciences University, Patna, Bihar 800014, India 4. Department of Veterinary Medicine, College of Veterinary Science and Animal Husbandry, Deen-dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-anusandhan Sansthan (DUVASU), Mathura, U.P. 281001 India 5. Department of Veterinary Physiology and Biochemistry, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University (NDVSU), Jabalpur, M.P., 483220 India 6. Veterinary Clinical Complex, Bihar Veterinary College, Bihar Animal Sciences University, Patna, Bihar 800014, India
Abstract
Cystic endometrial hyperplasia-pyometra complex (CEH-P) is a common disease in sexually mature bitches. Disease progression leads to oxidative stress, resulting in the depletion of uterine antioxidants and lipid peroxidation of associated cells, which further aggravates the condition. The concentration of antioxidant enzymes, the level of lipid peroxidation within the uterine tissue, and its reflection in the serum and urine need to be elucidated. The aim of this study was to analyze the concentration of antioxidants such as superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), glutathione peroxidase (GPx), and the lipid peroxidation marker malonaldehyde (MDA) in three types of samples, i.e., serum, urine, and uterine tissue. For this purpose, 58 pyometra-affected and 44 healthy bitches were included in the present study. All animals underwent ovariohysterectomy (OVH). Our data indicated highly significant difference (p<0.01) in the antioxidant concentrations of uterine, serum and urine samples. Furthermore, there was a highly significant (p<0.01) difference in the serum levels of ferric reducing antioxidant power (FRAP) and free radical scavenging activity (FRSA) indicated poor capacity to overcome oxidative stress in the CEH-Pyometra condition. We showed that CEH-P induces oxidative stress, which further depletes the antioxidant enzyme reserves in the uterus. Thus, the weak antioxidant defence predisposes to uterine damage and disease progression. The simultaneous depletion of antioxidants and an increase in lipid peroxidation in the serum and urine may also act as early indicators of uterine pathology.
Publisher
Polish Academy of Sciences Chancellery
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