Diagnostic significance of studying the crystallogenic properties of biological fluids in a child with a deficiency of 3-hydroxyacyl-CoA dehydrogenase

Abstract

Relevance: Crystalloscopy - a method for diagnosing biological fluids (BF) allows you to evaluate the physical properties of BF crystals after dehydration, as well as indirectly determine the state of the BF proteome. Crystallography helps to identify pathological processes (inflammation, oncogenesis, trauma) in the body and further monitor the effectiveness of treatment. A defect in the HAD enzyme leads to a sharp decrease in ketogenesis, the accumulation of long-chain fatty acids, an increase in the formation of dicarboxylic acids that negatively affect the tissues of the brain, heart and liver, and inhibition of a number of enzymes (in particular, gluconeogenesis enzymes). Objective: to conduct a comparative analysis of the results of crystalloscopic examination of biological fluids in normal and metabolic pathology. Materials. Materials and methods: Crystalloscopic examination of saliva of 20 people and urine of 7 examined children without a verified diagnosis associated with metabolic pathology and a child with an established pathology of enzyme systems with liver damage. For a detailed analysis of the results of the study, the case history of child B., 10 months old, hospitalized at the Institute of Pediatrics of the University Clinic of the Volga Research Medical University in 2022, was considered. Discussion: The crystallogenic activity of the biological fluids of a patient with congenital liver enzymopathy is significantly transformed. In dried micropreparations of urine, a moderate increase in the crystal-forming ability of the biomedium was recorded, as indicated by an increase in the level of crystallization and structural index. A sharp increase in the degree of destruction of crystalline structures in the facies testified to the pathological nature of the changes. Also, pronounced shifts in the crystallogenic properties were revealed in the crystallograms of the patient’s blood serum according to the activation of dehydration structurization of the blood serum, as indicated by an increase in crystallization and structure index. Similar to urine samples, a sharp increase in the degree of facies destruction was recorded in this biological fluid compared to samples from healthy children. Conclusions: The method of crystalloscopic examination of biological fluids has the potential in the diagnosis of congenital and acquired persistent metabolic disorders due to the information content and simplicity and cost-effective study within the framework of medical examination.