Affiliation:
1. Saratov State Medical University named after V.I. Razumovsky
Abstract
Purpose: to evaluate the quantitative and qualitative features of the metabolic activity of the intestinal microbiota in patients with obesity. Materials and methods: a comprehensive clinical, laboratory and instrumental examination of 60 children aged 12-16 years was carried out. The main group (n=30) - patients with exogenous constitutional obesity, the comparison group (n=30) - adolescents with normal body weight. The metabolic activity of the intestinal microbiota was assessed by gas-liquid chromatography of a fecal sample with the determination of the relative and absolute values of short-chain fatty acids (SCFAs). Results: All patients with obesity showed a decrease in the concentration of SCFA compared with the comparison group, which indicated a decrease in the metabolic activity of the obligate lactic acid flora and the predominance of the fraction of non-proteolytic anaerobic microorganisms. When assessing the relationship between the lipid spectrum and individual SCFAs and their indices, numerous positive relationships of moderate strength were obtained, which indirectly may indicate the progression of the imbalance of aerobes and anaerobes of the intestinal microbiome with an increase in the degree of metabolic disorders in obese patients. Conclusion: The identified qualitative and quantitative disorders of the intestinal microbiota in obese children are associated with the development of insulin resistance and are a reflection of metabolic endotoxinemia associated with the maintenance of chronic sluggish inflammation in overweight children.
Publisher
LLC Global Media Technology
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