Author:
Li Xiaoli,Lu Shenyi,Jiang Yan,Xu Kai,He Jingzhi,Qiu Zhangli,Ying Pian
Abstract
<b><i>Objectives:</i></b> The aim of our study was to explore the role of circular RNA_0061140 (circ_0061140) in adenomyosis progression and its associated mechanism. <b><i>Design:</i></b> We first analyzed the expression pattern of circ_0061140 in endometrial tissues of adenomyosis patients (<i>n</i> = 27) and uterine fibroid patients (<i>n</i> = 15). Loss-of-function experiments were conducted to analyze the biological roles of circ_0061140 in regulating the viability, apoptosis, proliferation, migration, and invasion of endometrial epithelial cells. The downstream microRNA (miRNA)/messenger RNA (mRNA) axis of circ_0061140 was predicted by bioinformatics tool Starbase, and its working mechanism was verified by rescue experiments. <b><i>Methods:</i></b> Cell viability, apoptosis, proliferation, invasion, and migration were assessed by cell counting kit-8 assay, flow cytometry analysis, 5-ethynyl-2′-deoxyuridine assay, transwell assay, and scratch test. The binding relationship between miR-141-3p and circ_0061140 or lin-28 homolog B (LIN28B) was verified by dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay. <b><i>Results:</i></b> Circ_0061140 expression was upregulated in adenomyosis patients. Circ_0061140 knockdown suppressed the viability, proliferation, invasion, and migration and triggered the apoptosis of endometrial epithelial cells. Circ_0061140 served as a miRNA sponge for miR-141-3p, and miR-141-3p silencing partly reversed circ_0061140 knockdown-induced effects in endometrial epithelial cells. miR-141-3p directly interacted with LIN28B mRNA. LIN28B overexpression partly diminished miR-141-3p overexpression-mediated influences in endometrial epithelial cells. Circ_0061140 knockdown downregulated LIN28B expression by elevating miR-141-3p level in endometrial epithelial cells. <b><i>Limitations:</i></b> The functional verification of circ_0061140/miR-141-3p/LIN28B axis was merely conducted in vitro. <b><i>Conclusion:</i></b> Circ_0061140 contributed to adenomyosis progression by binding to miR-141-3p to induce LIN28B expression in vitro.
Subject
Obstetrics and Gynecology,Reproductive Medicine