Song in an Affiliative Context Relates to the Neural Expression of Dopamine- and Neurotensin-Related Genes in Male European Starlings

Abstract

Some animals, including songbirds, vocalize at high rates when alone or in large groups. In songbirds, vocal behavior in these contexts is important for song learning and group cohesion. It is not obviously targeted at any particular individual and is described as ‘undirected'. Studies suggest a role for dopamine (DA) in undirected song. The neuropeptide neurotensin (NT) can enhance dopaminergic signaling upon binding to the NT receptor 1 (NTR1) and is found in regions where DA can influence song, including the ventral tegmental area (VTA), septum, and the song control nucleus Area X. To begin to test the hypothesis that NT and DA in these regions interact to influence undirected song, we used quantitative real-time PCR to relate undirected singing to mRNA expression of NT, NTR1, tyrosine hydroxylase (TH; a synthetic enzyme for DA) and D1 and D2 receptors in male European starlings. TH and NT expression in VTA, and NT and D1 expression in Area X, positively correlated with song. NT markers also correlated positively with DA markers in VTA. Given the role of VTA projections to Area X in song learning, these results suggest that interactions between NT and DA in these regions may contribute to vocal learning. In septum, NTR1 expression positively correlated with song and NT and DA markers were correlated, suggesting that NT in this region may influence dopaminergic transmission to facilitate undirected vocalizations. Overall, these findings implicate interactions between NT and DA in affiliative communication.