Role of Presurgical Gut Microbial Diversity in Roux-en-Y Gastric Bypass Weight-Loss Response: A Cohort Study

Abstract

<b><i>Introduction:</i></b> Roux-en-Y gastric bypass (RYGB) substantially alters the gut microbial composition which could be associated with the metabolic improvements seen after surgery. Few studies have been conducted in Latin American populations, such as Mexico, where obesity prevalence is above 30% in the adult population. Thus, the aim of this study was to characterize the changes in the gut microbiota structure in a Mexican cohort before and after RYGB and to explore whether surgery-related changes in the microbial community were associated with weight loss. <b><i>Methods:</i></b> Biological samples from patients who underwent RYGB were examined before and 12 months after surgery. Fecal microbiota characterization was performed through 16S rRNA sequencing. <b><i>Results:</i></b> Twenty patients who underwent RYGB showed a median excess weight loss of 66.8% 12 months after surgery. Surgery increased alpha diversity estimates (Chao, Shannon index, and observed operational taxonomic units, <i>p</i> &lt; 0.05) and significantly altered gut microbiota composition. Abundance of four genera was significantly increased after surgery: <i>Oscillospira, Veillonella, Streptococcus</i>, and an unclassified genus from <i>Enterobacteriaceae</i> family (<i>P</i>_FDR &lt; 0.1). The change in <i>Veillonella</i> abundance was associated with lower excess weight loss (rho = −0.446, <i>p</i> = 0.063) and its abundance post-surgery with a greater BMI (rho = 0.732, <i>p</i> = 5.4 × 10⁻⁴). In subjects without type 2 diabetes, lower bacterial richness and diversity before surgery were associated with a greater <i>Veillonella</i> increase after surgery (<i>p</i> &lt; 0.05), suggesting that a lower bacterial richness before surgery could favor the bloom of certain oral-derived bacteria that could negatively impact weight loss. <b><i>Conclusion:</i></b> Presurgical microbiota profile may favor certain bacterial changes associated with less successful results.