High-Level Resistance to Erythromycin and Tetracycline and Dissemination of Resistance Determinants among Clinical Enterococci in Iran

Author:

Ahmadpoor Nikta,Ahmadrajabi Roya,Esfahani Sarvenaz,Hojabri Zoya,Moshafi Mohammad Hassan,Saffari Fereshteh

Abstract

<b><i>Objectives:</i></b> The purpose of this study was to investigate the distribution pattern of genes responsible for erythromycin and tetracycline resistance and their association with resistance phenotypes in enterococcus isolates. <b><i>Materials and Methods:</i></b> Eighty-six <i>Enterococcus faecalis</i> and 26 <i>E. faecium</i> isolates were collected from 2 hospitals in Kerman, Iran. Minimum inhibitory concentration of erythromycin and tetra­cycline was determined and then genes encoding resistance to erythromycin – <i>erm</i> (A-C), <i>mef,</i> and <i>msr</i> – and tetracycline – <i>tet</i> (M), <i>tet</i> (O), <i>tet</i> (S), <i>tet</i> (K), and <i>tet</i> (L) – were investigated. <b><i>Results:</i></b> In all resistant isolates (<i>n</i> = 72, 64%), high-level resistance to both tested antibiotics was found. The most prevalent <i>erm</i> gene was <i>erm</i> (B) (77.7%), followed by <i>erm</i> (A) (15.2%) and <i>erm</i> (C) (8.3%). Genes mediating erythromycin efflux were detected in 70.8% (<i>mef</i>) and 9.7% (<i>msr</i>) of resistant isolates. Regarding tetracycline, <i>tet</i> (M) was detected at the highest rate (50%), followed by <i>tet</i> (O) (31%) and <i>tet</i> (S) (11%). Export of tetracycline was found in 31% (<i>tet</i> (K)) and 12% (<i>tet</i> (L)) of isolates. <b><i>Conclusion:</i></b> A high prevalence of high-level resistance to both erythromycin and tetracycline was documented. Alterations at the ribosomal level was more frequently detected in erythromycin and tetracycline resistance than efflux systems. Concurrent resistance mechanisms were more involved in resistance to erythromycin than tetracycline.

Publisher

S. Karger AG

Subject

General Medicine

Reference10 articles.

1. Choi J, Rieke EL, Moorman TB, Soupir ML, Allen HK, Smith SD, et al. Practical implications of erythromycin resistance gene diversity on surveillance and monitoring of resistance. FEMS Microbiol Ecol. 2018 Apr;94(94):1–11.

2. Huys G, D’Haene K, Collard JM, Swings J. Prevalence and molecular characterization of tetracycline resistance in Enterococcus isolates from food. Appl Environ Microbiol. 2004 Mar;70(70):1555–62.

3. Saffari F, Dalfardi MS, Mansouri S, Ahmadrajabi R. Survey for correlation between biofilm formation and virulence determinants in a collection of pathogenic and fecal Enterococcus faecalis isolates. Infect Chemother. 2017 Sep;49(3):176–83.

4. Rathnayake IU, Hargreaves M, Huygens F. Antibiotic resistance and virulence traits in clinical and environmental Enterococcus faecalis and Enterococcus faecium isolates. Syst Appl Microbiol. 2012 Jul;35(35):326–33.

5. Arabestani MR, Nasaj M, Mousavi SM. Correlation between infective factors and antibiotic resistance in enterococci clinical isolates in west of Iran. Chonnam Med J. 2017 Jan;53(53):56–63.

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3