Role of Nitric Oxide in the Change of 5-Hydroxytryptamine Synthesis in the Intestine by a Consecutive Administration of Methotrexate to Rats-Reference-Cited by-同舟云学术

Role of Nitric Oxide in the Change of 5-Hydroxytryptamine Synthesis in the Intestine by a Consecutive Administration of Methotrexate to Rats

Published:2020 Issue:11-12 Volume:105 Page:723-728
ISSN:0031-7012
Container-title:Pharmacology
language:en
Short-container-title:Pharmacology

Author:

Machida Takuji,Inotani Akari,Shiga Saki,Kon Shuto,Yanada Takumi,Kobayashi Hiroya,Hamaue Naoya,Hirafuji Masahiko,Iizuka Kenji

Abstract

This study aimed to investigate whether the consecutive administration of methotrexate affects 5-hydroxytryptamine (5-HT) synthesis in the rat small intestine. Rats received methotrexate at a dose of 12.5 mg/kg intraperitoneally on 4 consecutive days. <i>N</i><sup>G</sup>-nitro-L-arginine methyl ester (L-NAME) was given subcutaneously to inhibit nitric oxide (NO) synthase. Methotrexate moderately altered 5-HT synthesis, whereas the combined administration of methotrexate and L-NAME significantly changed 5-HT synthesis in the rat ileal tissue. These results suggest that endogenous NO has an antagonistic role in the induction of 5-HT synthesis in rats following the consecutive administration of methotrexate.

Publisher

S. Karger AG

Subject

Pharmacology,General Medicine

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