The Effect of Solutions Containing Extracts of Vochysia tucanorum Mart., Myrcia bellaCambess., Matricaria chamomilla L. and Malva sylvestris L. on Cariogenic Bacterial Species and Enamel Caries Development

Abstract

This study evaluated the effect of experimental solutions containing plant extracts on bacterial species and enamel caries prevention. Microcosm biofilm was produced from human saliva mixed with McBain saliva (0.2% sucrose) on bovine enamel for 5 days (3 days under anaerobiosis and 2 days under aerobiosis) at 37°C. From the 2nd day, the following treatments were applied (1 × 60 s/day): <i>Vochysia tucanorum</i> (10 mg/mL); <i>Myrcia bella</i> (5 mg/mL); <i>Matricaria chamomilla</i> (80 mg/mL); <i>Malva sylvestris</i>, fluoride, and xylitol (Malvatricin Plus®); 0.12% chlorhexidine (CHX, PerioGard®); and PBS (negative control). The medium pH was measured. Quantitative polymerase chain reaction was performed for the detection of <i>Streptococcus mutans</i> and <i>Lactobacillus</i> spp. Enamel demineralization was measured by spectral-domain optical coherence tomography. The data were compared by means of the Kruskal-Wallis/Dunn, two-way ANOVA/Bonferroni, and ANOVA/Tukey tests (<i>p</i> &#x3c; 0.05). The pH decreased after sucrose exposure; only CHX reestablished pH &#x3e;5.5 by the last day. CHX also eliminated <i>Lactobacillus</i>spp., but the other treatments did not differ significantly from PBS. Malvatricin Plus® and CHX eliminated <i>S. mutans</i>, but the other treatments did not differ from PBS. Similar results were seen concerning the reduction of lesion depth and reflectivity. The experimental natural-extract solutions were ineffective against cariogenic bacteria and in preventing the development of enamel caries.