A Review of Conventional PCR Assays for the Detection of Selected Phytopathogens of Wheat

Abstract

Infection of phyllosphere (stems, leaves, husks, and grains) by pathogenic fungi reduces the wheat yield and grain quality. Detection of the main wheat pathogenic fungi provides information about species composition and allows effective and targeted plant treatment. Since conventional procedures for the detection of these organisms are unreliable and time consuming, diagnostic DNA-based methods are required. Nucleic acid amplification technologies are independent of the morphological and biochemical characteristics of fungi. Microorganisms do not need to be cultured. Therefore, a number of PCR-based methodologies have been developed for the identification of key pathogenic fungi, such as <i>Fusarium</i> spp., <i>Puccinia</i> spp., <i>Zymoseptoria tritici</i>, <i>Parastagonospora nodorum</i>,<i> Blumeria graminis </i>f. sp.<i> tritici</i>, and<i> Pyrenophora tritici-repentis</i>. This article reviews frequently used DNA regions for fungus identification and discusses already known PCR assays for detection of the aforementioned wheat pathogens. We demonstrate that PCR-based wheat pathogen identification assays require further research. In particular, the number of diagnostic tests for <i>Fusarium graminearum</i>, <i>Puccinia</i> spp., and <i>P. tritici-repentis</i> are insufficient.