Abstract
This study aimed to determine the effects of systemically administered hydroxytyrosol (HT) on alveolar bone resorption and oxidative stress parameters in experimental periodontitis (EP). Thirty-two rats were divided randomly into four groups; 1) periodontally healthy + serum physiologic (PH-SP), 2) PH + hydroxytyrosol (PH-HT), 3) experimental periodontitis + SP (EP-SP), and 4) EP-HT. Following induction of EP, 10 mg/kg of systemic HT (test)/SP (control) was administered and continued for 14 days. The animals were euthanized on the 15th day, and the jaws were removed for histopathologic, histomorphometric, and immunohistochemical analyses. Enzyme-linked immunosorbent assay (ELISA) was used to analyze serum and gingival tissue malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) levels. The receptor activator of NF-kB ligand (RANKL), osteoprotegerin (OPG) levels, and RANKL/OPG ratio were analyzed via immunolabeling. Serum and tissue MDA, SOD, and GSH-Px levels did not differ between the groups. The immunohistochemical evaluation showed that RANKL levels and RANKL/OPG ratio in HT applied groups were significantly lower than SP applied groups. Within the limits of this study, daily administration of hydroxytyrosol at a dose of 10 mg/kg for 14 days could prevent alveolar bone destruction in experimental periodontitis. Besides, the antioxidant effect of HT could not be revealed.