Evaluation of the association of anti-thyroid peroxidase with antinuclear antibodies and different antinuclear antibodies patterns

Abstract

Aims: To investigate the relationship between anti-thyroid peroxidase (TPO), a marker for Hashimoto’s thyroiditis, and antinuclear antibodies (ANA) and ANA patterns, biomarkers for systemic autoimmune diseases. Methods: In this study, ANA and anti-TPO test results, obtained in our hospital laboratory between 2019 and 2022, were retrospectively evaluated. ANA was detected by the indirect immunofluorescence antibody method using commercial HEp-20-10 cell substrates and anti-TPO was determined by the sandwich immunoassay method using a commercial immunoassay analyzer. Results: A total of 1750 patients’ results were analyzed. ANA was positive in 28.7% of anti-TPO-positive patients and 19% of anti-TPO-negative patients. While 6.4% (112/1750) of patients were positive for both ANA and anti-TPO, both test results were negative in 62.85% of patients (p