Masculinization of betta fish Betta splendens by embryo immersion in extract of purwoceng Pimpinella alpina

Abstract

<p class="NoParagraphStyle" align="center"><strong>ABSTRACT</strong></p><p class="NoParagraphStyle" align="center"> </p><p class="NoParagraphStyle">This study aimed to examine the effect of extract of purwoceng <em>Pimpinella alpina</em> for masculinization of <em>Betta splendens</em>. This research used completely randomized design with three treatments that were distinguished by doses of purwoceng extract, which were 10, 20, and 30 µL/L, and 0 µL/L as control. The treatments were given by immersion to 35 embryos at eye spots phase or about 28-hours after fertilization, for eight hours. The immersion process was done for 8 hours. The result showed that 20 µL/L dose of purwoceng extract produced 62.66% male, and hatching rate was 85.71%. This male percentage was higher compared to control (45.91%), but the hatching rate was lower than that of control (98.57%). At the higher dose (30 µL/L), male fish population and hatching rate reduced respectively 39.72% and 68.57%, respectively.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Keywords: masculinization, embryo immersion, purwoceng extract, <em>Betta splendens</em></p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle" align="center"><strong>ABSTRAK</strong></p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Penelitian ini bertujuan untuk mengevaluasi pengaruh dosis ekstrak purwoceng <em>Pimpinella alpina</em> extract dalam maskulinisasi ikan cupang hias <em>Betta splendens</em>. Penelitian ini menggunakan metode rancangan acak lengkap dengan tiga perlakuan dan tiga ulangan dosis ekstrak purwoceng. yaitu 10, 20, dan 30 µL/L, serta perlakuan 0 µL/L sebagai kontrol. Perlakuan diberikan melalui perendaman selama delapan jam terhadap 35 embrio saat memasuki fase bintik mata atau sekitar jam ke-28 setelah pembuahan. Hasil penelitian menunjukkan bahwa dosis ekstrak purwoceng 20 µL/L menghasilkan persentase ikan jantan sebesar 62,66%, dan daya tetas telur sebesar 85,71%. Persentase jantan tersebut lebih tinggi daripada kontrol (45,1%), tetapi derajat penetasannya lebih rendah daripada kontrol (98,57%). Pada dosis yang lebih tinggi (30 µL/L), populasi ikan jantan (39,72%), dan derajat penetasan (68,57%) adalah menurun.</p><p class="NoParagraphStyle"> </p><p>Kata kunci: maskulinisasi, perendaman embrio, ekstrak purwoceng, <em>Betta splendens</em></p>