Isolation and diagnosis of some associated fungi with cowpea root rot disease and testing its pathogenicity

Abstract

Execute search by date 1/4/2021, The results of collecting samples from the regions of Anbar, Baghdad, Salah al-Din and Wasit showed that cowpea root rot disease is widespread in all studied areas, and The results of isolation phenotypic and molecular diagnosis showed the presence of different isolation of fungi that infected cowpea root, such as Fusarium nygamai (Fu1), F. nygamai (Fu2), F. solani (Fu3), F. solani(Fu4), Rhizoctonia solani (Rh5), and Fusarium solani (Fu6), The results of the pathogenicity test on radish seeds showed that tested isolates were significantly decreased germination percentage of radish seeds of water Ager, and the most effective isolation was F. solani (Fu4). The infection rate was 90% compared to 0.00% of the control media treatment, which was uncontaminated by the pathogenic fungus. Isolated fungi showed a difference in the percentage and severity of infection on cowpea seedlings and seedlings, as the isolate of F.nygamai (Fu1) achieved the highest infection rate of 66.67 % and the severity of disease at 75%. All fungal isolates significantly increased the rate and severity of infection on seed radish compared with the control treatment not contaminated with pathogenic fungi by 0.0%. Keywords: Vegan unguiculata; Rhizoctonia solani; and Fusarium solani; PCR.