Structural analysis and cytotoxic evaluation of kisspeptin10 and analogs in types of cancer

Abstract

The Kisspeptin system is a peptidergic system that plays a crucial role in regulating of reproduction and hormonal function. Kisspeptin is a peptide synthesized from the KiSS-1 gene and has been identified as the endogenous ligand of the kisspeptin receptor (KISS1R or GPR54 receptor). This system plays a key role in activating sex hormone secretion and puberty. In addition to its function in the regulation of reproduction, the Kisspeptin system has been found to play a role in other physiological processes, such as the regulation of appetite, energy metabolism, cardiovascular function, and cancer. In this study, several Kisspeptin analogs with structural modifications were designed and synthesized. The Kisspeptin analogs were evaluated by in vitro cytotoxicity tests on cancer cells of different cancer types. Cell viability assays were performed, and the concentrations that inhibited cell growth by a significant percentage were determined. The results showed that certain Kisspeptin analogs exhibited increased selective cytotoxicity in cancer cells compared to healthy cells. In conclusion, this study demonstrates that structurally modified Kisspeptin analogs have the potential to be therapeutic agents against some types of cancer. Understanding the structure-activity relationship of these analogs and their evaluation of their selective toxicity on cancer cells will be of great importance. Keywords: Kisspeptins Analogs, GPR54, Cancer, Cytotoxicity, Molecular Docking, Structure-activity relationship, Anticancer therapy, Drug Design.