Molecular detection of Escherichia coli efflux pumps genes isolated from UTI in pregnant women

Abstract

Sixty-three clinical samples from midstream urine samples were collected from pregnant women with urinary tract infections. After microscopic examination, culture and biochemical tests and the final diagnosis using the VITEK-2 system, 25 Escherichia coli isolates were discovered. Antimicrobial susceptibility results showed that E.coli isolates were resistant to gentamicin (%92), amikacin (%28), norfloxacin (%52), Ciprofloxacin (%56), ofloxacin (%60), trimethoprim (%8), chloramphenicol (%80), colistin sulfate (%20), tetracyclin (%68), azithromycin (%48), cefoxitin (%40), amoxicillin-clavulanate (%96), ampicillin (%92). The prevalence of capsule posses, hemolysin production, biofilm formation, and efflux pumps wrer%24,%16,%72 and %44 respectively. The result of efflux pumps genes acrA and acrB gene detection was 100. The acrA and acrB gene expression increased after treatment with the antibiotic Ciprofloxacin. Because of the primary role of Escherichia coli in urinary tract infection and the presence of a high ratio of Multidrug Resistance ( MDR ), and the importance of The efflux pumps in antibiotics resistance, the current study was conducted to determine the MDR isolates from UTI in pregnant women's in Baquba city, the percent of acrA and acrB genes among strains and the effect of Ciprofloxacin treatment on gene expression. Keywords. Escherichia coli, efflux pumps, acrA gene, acrB gene