Effect of aqueous extract of Azadirachta indica leaves on gastrointestinal nematodes in ruminants: an in vitro study-Reference-Cited by-同舟云学术

Effect of aqueous extract of Azadirachta indica leaves on gastrointestinal nematodes in ruminants: an in vitro study

Published:2023-09-15 Issue:3 Volume:8 Page:1-10
ISSN:1390-9347
Container-title:Bionatura
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Author:

Correa Herrera Nelson¹^ORCID,Martínez Montero Marcos Edel¹^ORCID,Cepero Rodríguez Omelio¹^ORCID,Jumbo Romero Manuel de Jesús²,Linares Rivero Claudia³^ORCID,Fontes Marrero Dayami¹^ORCID,Cuello Portal Sandra⁴^ORCID,Quiñones-Galvez Janet⁵^ORCID,Rodríguez Benalcázar Viviana Marina⁶^ORCID,Borja Ramos Alva Tatiana⁷

Affiliation:

1. Facultad de Ciencias Agrícolas, Universidad de Ciego de Ávila Máximo Gómez Báez, Cuba

2. Universidad Laica Eloy Alfaro de Manabí, Extensión en El Carmen

3. Centro de Bioplantas, Universidad de Ciego de Ávila Máximo Gómez Báez, Ciego de Ávila, Cuba

4. Centro Nacional de Sanidad Agropecuaria, Universidad Agraria de la Habana, Cuba

5. Centro de Bioplantas, Universidad de Ciego de Ávila Máximo Gómez Báez, Ciego de Ávila, Cuba.

6. Laboratorio de Microbiología. Departamento de Medicina Veterinaria, Facultad de Ciencias Agropecuarias, Universidad Técnica de Ambato

7. Laboratorio de Biología, Departamento de Medicina Veterinaria, Facultad de Ciencias Agropecuarias, Universidad Técnica de Ambato.

Abstract

High rates of gastrointestinal parasite infestation lead to the frequent use of anthelmintics for their control, resulting in resistance in the main species of gastrointestinal nematodes. Natural plant extracts, particularly those from Azadirachta indica A. Juss leaves, have been described as having antiparasitic, antimicrobial, insecticidal, antioxidant, and anticorrosive activities, which are related to the diversity of their chemical composition. This research aimed to evaluate the effect of the aqueous extract of A. indica leaves on the control of gastrointestinal nematodes in ruminants. To achieve this, an in vitro test was performed using the larval development assay, with fecal samples obtained from Pelibuey sheep, Criolla goats in the development category, and Criolla calf cattle for the culture of gastrointestinal nematode larvae. Three concentrations (minimum, medium, and maximum) were evaluated for each animal species against Haemonchus placei and Cooperia sp larvae for cattle and Haemonchus contortus, Trichostrongylus sp, and Trichuris for sheep and goats. The survival percentage indicator was evaluated at 0, 24, 48, and 72 h. The research concluded that the minimum concentration was valid for sheep and goats but not for cattle, and the medium and maximum concentrations were effective against all three animal species, resulting in 100% larval mortality. Keywords: Azadirachta, drug effects, Ruminants, Trichostrongyloidea

Publisher

Clinical Biotec

Subject

Infectious Diseases,Applied Microbiology and Biotechnology,Epidemiology,Biotechnology

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