Evaluation of humoral immunity by IgG measurement in broilers vaccinated with life and killed Newcastle vaccine
Author:
Talib Douaa Y.1, Thwiny Hazim T.2
Affiliation:
1. Department of Animal Production, College of Agriculture, University of Sumer, Thi Qar, Iraq 2. Department of Microbiology, College of Veterinary Medicine, University of Basrah, Basrah, Iraq
Abstract
Current research objectives evaluated the humeral immunity induced by two different vaccination methods and estimated the efficiency of the commercially available Newcastle disease (ND) vaccine. A total number of 90-day-old unvaccinated chicks were divided equally into 3 groups; each group was vaccinated with different vaccination methods, while group 3 was the unvaccinated control group. Serum was collected from all groups, and five chickens from each group were slaughtered. ELISA measured the levels of IgG; there also were higher in the vaccinated groups (G1, G2) compared with the unvaccinated group. Group 2 also had the best IgG; The higher values of lymphoid organs (Bursa of Fabricius, thymus and spleen) indices were in. While the vaccinated groups were compared to non-vaccinated groups, between vaccinated groups, there was no significant difference (P < 0.05). The best-proven vaccine program in group 2(primary vaccine at 7th day of old by the intraocular vaccine (live vaccine), then boost potion dose at 21st day of old by drinking water vaccine(live vaccine). Keywords: chicks, humeral immunity, vaccine.
Publisher
Clinical Biotec
Subject
Infectious Diseases,Applied Microbiology and Biotechnology,Epidemiology,Biotechnology
Reference19 articles.
1. 1. Zhu, W., Dong, J., Xie, Z., Liu, Q. and Khan, M.I. Phylogenetic and pathogenic analysis of Newcastle disease virus isolated from house. 2010;11456-062016-Reg. 689–698. 2. 2. Alexander, D. J. Newcastle disease and avian paramyxovirus infections, p. 541–569. In B. W. Calnek, H. J. Barnes, C. W. Beard, and L. R. McDougald (ed.), Diseases of poultry, 10th ed. Iowa State University Press, Ames, Iowa. 1997. 3. 3. Farhan, S. M., Abdulateef, S. M. Al-Enzy, A. F. M, Mohammed, Th. T., Saeid, Z. J. M., Al-Khalani, F. M. H. & Abdulateef, F. M. Effect of heat stress on blood alkalinity of broiler chicks and its reflection in improving the productive performance. Indian Journal of Ecology, 2020; 47: 107-109. 4. 4. Miller, P.J, Koch, G.Newcastle disease. In: Swayne, D. E., Glisson, J.R, McDouglad , L.R, Nolan , L.K, Suarez , D.L, Nair, V(E ds.)Disease of Poultry .Wiley –Blackwell, Hoboken , New Jersey , 2013; pp.89-138. http://refhub.elsevier.com/S0378-1135(16)30804-5/sbref0290 5. 5. Marangon, S. and Busani L. The use of vaccination in poultry production. Rev. Sci. Tech., Off. Int. Epiz., 2006; 26 (1): 265-274
|
|