Affiliation:
1. V.N. Karazin Kharkiv National University, Kharkiv, Ukraine
Abstract
Liver diseases cause many deaths worldwide and wreak havoc on the economy. The main hepatoprotectors are plant substances and peptides. Regenerative medicine based on mesenchymal stem cells (MSCs) can offer a new set of biologically active substances for liver regeneration, which are part of their secretome. The work applied the methods of cell isolation and cultivation, ultrafiltration for fractionation of secretome components, and organotypic culture model. The study’s results indicate an increase in the regenerative capacity of the liver under the impact of the components of the MSC secretome; the fraction below 10 kDa shows the most significant activity.
Keywords: organotypic liver culture; xenogenic stem cells; fractions of secretome; absorption spectrum, ultrafiltration
Subject
Infectious Diseases,Applied Microbiology and Biotechnology,Epidemiology,Biotechnology
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