Study of HLA-G gene polymorphism and serum level of soluble HLA-G in patients infected with Mycobacterium tuberculosis

Abstract

Tuberculosis affects about one-third of the world population. The incidence of the disease differs significantly among populations living under almost similar conditions, indicating the role of genetic factors. The present study aimed to appraise the impact of HLA-G gene polymorphisms and soluble HLA-G on the susceptibility to pulmonary tuberculosis. 48 patients with pulmonary tuberculosis and other 42 age- and sex-matched healthy individuals were included in the study. Both groups evaluated two gene polymorphisms in the HLA-G gene and soluble HLA-G protein. The frequency of Del/Del genotype of rs66554220 (14-bp Ins/Del) polymorphism in patients was 8.33% which was higher than that of controls (2.38%) with a significant difference (crude OR= 9.26, 95%CI=1.06-80.93, p=0.044). Such association remained significant after adjusting for confounding factors, including smoking, family history, socioeconomic status and residence (adjusted OR= 11.83, 95%CI=2.77-50.51, p= 0.01). The median serum level of soluble HLA-G in patients was 34.0 ng/ml (range 6.18-74.25 ng/ml), which was greater than that of controls (median 20 ng/ml, range 312-71.98 ng/ml) with a significant difference. We can conclude that The Del/Del genotype of rs66554220 (14-bp Ins/Del) polymorphism is an independent risk factor for pulmonary tuberculosis in the Iraqi population Keywords: Mycobacterium tuberculosis, HLA-G gene, single nucleotide polymorphism