Effects of Leptin antagonist treatments on testosterone and testis histological characteristics of immature male mice

Abstract

The present study aimed to ascertain how leptin antagonist injection affected testis weights, testis morphology and testosterone levels in immature male Swiss mice. Animals were administered with anti-leptin antibody subcutaneously, with or without equine chorionic gonadotropin (eCG). Control animals were treated with non-immune serum. Blood and testis were collected. The Androgen profile was analyzed in serum and tissue homogenates, and testes were histologically examined. Compared to controls, mice treated with an anti-leptin antibody with or without gonadotropins had a significant (p<0.05) increase in testis weight. Testosterone concentrations in the testis were significantly (p<0.05) higher in mice administered with anti-leptin antibody compared to control, but testosterone concentrations in blood were not affected. The diameter of seminiferous tubules, the diameter of the lumen and the width of spermatogenic cells were significantly (p<0.05) higher in mice in treatment groups compared to controls. We conclude that anti-leptin antibody administration in immature male mice increased testosterone concentrations in the testis and improved testis histological characteristics. Keywords: leptin; mouse; histology; testis; testosterone; immature male