Identification of the parasites that cause children's cutaneous leishmaniasis and several types of immune response cells

Abstract

Cutaneous leishmaniasis has received many epidemiological, diagnostic, therapeutic, quantitative and other studies. The current study aims to determine the type of leishmaniasis parasite that causes cutaneous leishmaniasis in children in the Al-Diwaniyah governorate in central Iraq to ​​assess the level of variations in globulins and immune cells in children with cutaneous leishmaniasis. Fifty-nine was a positive sample for the traditional laboratory examination of cutaneous leishmaniasis in children whose ages ranged between (8 months-10 and years). They were collected from the Maternity and Children Hospital from January 2020 to December 2021. The samples were subjected to molecular diagnostics to determine the type of leishmaniasis that causes the disease using Real-Time PCR. ELISA was approved to measure the levels of variation in the concentrations of IgG and IgM among the group of infected and healthy children. The results indicated that children's predominant type causing cutaneous leishmaniasis is L. major 52 samples (88.13%). The immunological study showed clear and statistically significant differences in the mean globulin concentrations for comparing the patient's L. major with the control group at a probability level (P≤0.05). The current study concluded that L. major is the dominant species responsible for cutaneous leishmaniasis. Keywords: Catenous leishmaniasis, L. major, Real-Time PCR.