Assessment of genetic fidelity of lacy tree philodendron (Philodendron bipinnatifidum Schott ex Endl.) micro propagated plants-Reference-Cited by-同舟云学术

Assessment of genetic fidelity of lacy tree philodendron (Philodendron bipinnatifidum Schott ex Endl.) micro propagated plants

Published:2022-02-15 Issue:1 Volume:7 Page:1-5
ISSN:1390-9347
Container-title:Bionatura
language:
Short-container-title:RB

Author:

Alwahibi Mona S.¹,Alhussein Alawaadh Asma¹,Hassan Dewir Yaser²^ORCID,Soliman Dina A.¹,Kadry Seliem Mayada³

Affiliation:

1. Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11495, Saudi Arabia

2. Plant Production Department, College of Food and Agriculture Sciences, King Saud University, Riyadh 11451, Saudi Arabia. 3Department of Horticulture, Faculty of Agriculture, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt.

3. Ornamental and Floriculture Department, Horticulture Research Institute, Antoniades, Alexandria 21599, Egypt

Abstract

Tissue culture is a potent means for producing clonally propagated plants. However, genetically identical regenerants are of great concern due to their economic consequences. Therefore, polymerase chain reaction (PCR)-based molecular markers are employed to detect somaclonal variations. In this study, the genetic fidelity of in vitro raised Philodendron bipinnatifidum clones, and their mother plant was tested using 11 randomly amplified polymorphic DNA (RAPD) markers. The RAPD decamers produced 92 amplicons with 8.4 bands ranging from 260-5000 bp. The bands varied from one to fifteen for primer 8 and primer 7, respectively. The genetic similarity between the micro propagated plantlets and mother plant of P. bipinnatifidum was nearly 100%, assuring uniformity and true-to-type regenerated plantlets for this commercially crucial ornamental plant.

Publisher

Clinical Biotec

Subject

Infectious Diseases,Applied Microbiology and Biotechnology,Epidemiology,Biotechnology

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