Addition of acai fruit extract to Tris extender and its effect on some parameters of ram semen preserved at cooling.

Abstract

This study was conducted in the animal field and laboratories of the Department of Animal Production / College of Agricultural Engineering Sciences / University of Baghdad from November 15, 2021 until April 25, 2022. Three local Awassi rams were used in this experiment, their ages were between 2.5 - 3 years, and they weighed between 50-58 kg, and semen was collected from them using an artificial vagina to obtain one ejaculate from each ram weekly. The samples were divided using a Tris extender, as follows: 1.0, 1.5, 2.0, 2.5 mg Acai extract / 1 ml Tris in addition to the control group. Measurements of AST, ALT and MDA concentrations were performed in the seminal plasma. The results of the current study showed that it had no significant effect on the concentration of MDA, AST and ALT in seminal plasma for the two periods ( 0 and 72 hours) of preservation for treatments T1, T2, T3 and T4, although the concentration of ALT enzyme and the concentration of MDA in the seminal plasma of T3 treatment were mathematically lower than the rest of the treatments in the two periods (0 and 72 hours), while there was an arithmetic decrease in the concentration of AST enzyme for treatment T4 over the rest of the treatments in the period (0 hours) and treatment T1 over the rest of the treatments after (72 hours) of preservation. Keywords: Acai fruit extract, semen, ram, preservation, 5 °C.