FSCN1 Gene Expression Influence on Breast Cancer in Iraqi Patients

Abstract

Our investigation aim was to ascertain how the Fascin actin-bundling protein 1 (FSCN1) gene affected breast cancer patients and how it affected the molecular type of the disease (Luminal A, Luminal B, Triple negative, Enrich Her2).By collecting blood samples from sixty patients with Breast cancer from many hospitals in Baghdad/Iraq between the periods (October 2021 to August 2022) and collecting forty volunteers heaths as Control. The volunteer's blood samples were collected and tested with Cancer antigen 15-3 (CA 15-3) to confirm absent breast cancer. The blood and control samples from these patients were collected, the RNA was extracted, and molecular methods using PCR and primers targeting the FSCN1 gene were used. The results showed that genes found in all patient samples have overexpression compared to healthy volunteers, and we also discovered differences in expression between the molecular types of breast cancer. We discovered that Triple-negative breast cancer is more affected than Luminal A, B, and Enrich Her2. Keywords: Breast Cancer, Molecular classification breast cancer, Fascin Actin Bundling protein 1 genes