Analyzing the Impact of a Formula Including a Partial Purified Aspergillus Niiger Protease

Abstract

Aspergillus niger protease has attracted the attention of experts in environmental biotechnology since fungi can grow on low-cost substrates and secrete vast quantities of the enzyme into the culture medium. In this study, the samples of Aspergillus niger were collected from bread and onion and then identified and screened for protease production using skim milk agar. The production of media has followed components in gm/100 ml –casein 1 g; yeast extract 0.6 g; urea 0.6 g; NH4H2PO4 0.6 g; (NH4)2SO4 0.6 g; NH4Cl 0.6g; DDW 100 ml; glucose 1 gm; and pH 8.5 for protease production, the produced protease in crud extract was partial purified with solid (NH4)2SO4 was added to it by continuous mixing on ice bag (80% saturation) and the protease activity was 640 AU/ml with specific activity of 1280 AU/mg. The formula containing the partially purified protease was prepared. The protease produced from A. niger applied in the formula was investigated by using skim milk agar, wells containing the protease-containing formula showed a significant clear zone, and this ensures the activity of this formula. Keywords: Protease; Skim milk agar; Iraq.