Affiliation:
1. Medical Microbiology, College of Medical Technology, AL-Kitab University, Kirkuk, Iraq
Abstract
Sewage uses wastewater consisting of human excreta, wash waters, and industrial
and agricultural wastes (e.g., wastes from livestock, i.e., chicken, cattle, and
horse) that enter the sewage system. The study aims to study bacteria isolated
from sewage water to know their source and different antibiotics used and compare it with other bacteria present in human excretion. This study was carried out
in March - 2019. 30) Water samples were taken from the Al-Khassa River in
Kirkuk, starting from the Al-Tabaqchary bridge to the Domiez bridge; all the
samples were worked in the general health laboratory of Kirkuk. All the water
samples were collected in sterile containers and were processed within one hour
after arriving at the laboratory. First, it was cultured on blood and MacConkey
agar for each sample; the petri dishes were put in an aerobic incubator at 37 C0 for
24 hours. It was isolated on nutrient agar for purification, incubating at 37 C0 for
24 hours. Bacterial colonies were identified based on diagnosis using morphology, cultured, and biochemical characteristics. The antimicrobial screening was
performed using Muller-Hinton agar disk diffusion assay for signing drug sensitivity; the study showed that E. coli represented the most isolated bacteria from
Al-Khassa River (14 isolates), Serratia marcescens (10 isolates), Pseudomonas
aeruginosa (5 isolates) and Proteus spp. (only 2 isolates), The study showed that
gentamicin was the most active antibiotic against the Gram-negative bacilli isolates tested, as only 2.6% of these isolates were resistant to this antibiotic at concentrations up to 10μg while susceptible to clindamycin.
Keywords: Al-Khassa; Kirkuk; Sewege, river; Contamination
Subject
Infectious Diseases,Applied Microbiology and Biotechnology,Epidemiology,Biotechnology
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