Virome of ancient wolf mummy (<i>Canis lupus</i>) as a potential source of virulent bacteriophages for phage therapy-Reference-Cited by-同舟云学术

Virome of ancient wolf mummy (<i>Canis lupus</i>) as a potential source of virulent bacteriophages for phage therapy

Published:2023-04-13 Issue:1 Volume: Page:35-37
ISSN:1609-1175
Container-title:Pacific Medical Journal
language:
Short-container-title:Tihookeanskij medicinskij žurnal

Author:

Goncharov A. E.¹,Ahremenko Ya. A.²^ORCID,Azarov D. V.³,Cheprasov M. J.²,Fedorov S. E.²,Solomenny A. P.⁴,Kolodzhieva V. V.⁵

Affiliation:

1. Institute of Experimental Medicine; North-Western State Medical University named after I.I. Mechnikov; Saint-Petersburg State University

2. M.K. Ammosov North-Eastern Federal University

3. Institute of Experimental Medicine; North-Western State Medical University named after I.I. Mechnikov

4. Institute of Ecology and Genetics of Microorganisms of RAS Ural Branch

5. North-Western State Medical University named after I.I. Mechnikov

Abstract

Aim. To investigate the potential for searching new virulent bacteriophages in the paleontological material extracted from the permafrost zone.Material and methods. The virome structure of the colon content of the wolf pup mummy (Canis lupus) from the late Pleistocene was evaluated by means of shotgun metagenomic sequencing.Results. The study demonstrated the predominance of Myoviridae tailed bacteriophages, including PhiKZ-like phages, in the structure of the virome.Conclusion. The results of the study indicate the possibility of using paleontological material preserved in the ancient Arctic permafrost as a resource for searching and isolating new virulent bacteriophages.

Publisher

Pacific State Medical University

Subject

General Medicine

Reference9 articles.

1. Kerfahi D, Tripathi BM, Dong K, Kim M, Kim H, Slik JF, Adams JM. From the high Arctic to the equator: do soil metagenomes differ according to our expectations? Microbial ecology, 2019;77(1):168–85. doi: 10.1007/s00248-018-1215-z

2. Zablocki O, van Zyl L, Adriaenssens EM, Rubagotti E, Tuffin M, Cary SC, Cowan DA. High-level diversity of tailed Phages, eukaryote-associated viruses, and virophage-like elements in the metaviromes of Antarctic soils. Appl Environ Microbiol 2014;80(22):6888–97. doi: 10.1128/AEM.01525-14

3. López-Bueno A, Tamames J, Velazquez D, Moya A, Quesada A, Alcami A High diversity of the viral community from an Antarctic Lake. Science 2009;326:858–61. doi: 10.1126/science.1179287

4. Grigoriev SE, Cheprasov MYu, Savvinov GN, Tikhonov AN, Novgorodov GP, Fedorov SE, Boeskorov GG, Protopopov AV, Plotnikov VV, Belolubsky IN, Protodiyakonov KE, Van der Plicht J. Paleontological and Archaeozoological Investigations in the Yana River Basin. Vestnik of the M.K. Ammosov North-Eastern Federal University. 2017;1(57):20–35 (in Russ.).

5. Danilova YA, Belousova VV, Moiseenko AV, Vishnyakov IE, Yakunina MV, Sokolova OS. Maturation of Pseudo-Nucleus Compartment in P. aeruginosa, Infected with Giant phiKZ Phage. Viruses. 2020;12:1197. doi: 10.3390/v12101197